Unmasking imaging forces on soft biological samples in liquids when using dynamic atomic force microscopy: a case study on viral capsids.
نویسندگان
چکیده
Dynamic atomic force microscopy is widely used for the imaging of soft biological materials in liquid environments; yet very little is known about the peak forces exerted by the oscillating probe tapping on the sample in liquid environments. In this article, we combine theory and experiments in liquid on virus capsids to propose scaling laws for peak interaction forces exerted on soft samples in liquid environments. We demonstrate how these laws can be used to choose probes and operating conditions to minimize imaging forces and thereby robustly image fragile biological samples.
منابع مشابه
Atomic force microscopy contact, tapping, and jumping modes for imaging biological samples in liquids.
The capabilities of the atomic force microscope for imaging biomolecules under physiological conditions has been systematically investigated. Contact, dynamic, and jumping modes have been applied to four different biological systems: DNA, purple membrane, Alzheimer paired helical filaments, and the bacteriophage phi29. These samples have been selected to cover a wide variety of biological syste...
متن کاملSensitivity Analysis of Frequency Response of Atomic Force Microscopy in Liquid Environment on Cantilever's Geometrical Parameters
In this paper, the non-linear dynamic response of rectangular atomic force microscopy in tapping mode is considered. The effect of cantilever’s geometrical parameters (e.g., cantilever length, width, thickness, tip length and the angle between the cantilever and the sample's surface in liquid environment has been studied by taking into account the interaction forces. Results indicate that the r...
متن کاملAtomic Force Microscopy Application in Biological Research: A Review Study
Atomic force microscopy (AFM) is a three-dimensional topographic technique with a high atomic resolution to measure surface roughness. AFM is a kind of scanning probe microscope, and its near-field technique is based on the interaction between a sharp tip and the atoms of the sample surface. There are several methods and many ways to modify the tip of the AFM to investigate surface properties, ...
متن کاملCompositional contrast of biological materials in liquids using the momentary excitation of higher eigenmodes in dynamic atomic force microscopy.
Atomic Force microscope (AFM) cantilevers commonly used for imaging soft biological samples in liquids experience a momentary excitation of the higher eigenmodes at each tap. This transient response is very sensitive to the local sample elasticity under gentle imaging conditions because the higher eigenmode time period is comparable to the tip-sample contact time. By mapping the momentary excit...
متن کاملOrigins of phase contrast in the atomic force microscope in liquids.
We study the physical origins of phase contrast in dynamic atomic force microscopy (dAFM) in liquids where low-stiffness microcantilever probes are often used for nanoscale imaging of soft biological samples with gentle forces. Under these conditions, we show that the phase contrast derives primarily from a unique energy flow channel that opens up in liquids due to the momentary excitation of h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Biophysical journal
دوره 95 5 شماره
صفحات -
تاریخ انتشار 2008